ARG66996
anti-PERK antibody
anti-PERK antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PERK |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PERK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to Human PERK. |
| 偶联标记 | Un-conjugated |
| 別名 | PRKR-like endoplasmic reticulum kinase; PERK; HsPEK; Eukaryotic translation initiation factor 2-alpha kinase 3; Pancreatic eIF2-alpha kinase; WRS; PEK; EC 2.7.11.1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 130-140 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NZJ5 Human Eukaryotic translation initiation factor 2-alpha kinase 3 |
|---|---|
| 基因名称 | EIF2AK3 |
| 全名 | eukaryotic translation initiation factor 2-alpha kinase 3 |
| 背景介绍 | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015] |
| 生物功能 | Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity). [UniProt] |
| 预测分子量 | 125 kDa |
| 翻译后修饰 | Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity). Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. N-glycosylated. ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG66996 anti-PERK antibody IHC-p image
Immunohistochemistry: Formalin-fixed and paraffin-embedded human cancer tissue section tained with ARG66996 anti-PERK antibody.
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ARG66996 anti-PERK antibody ICC/IF image
Immunofluorescence: Formalin-fixed Raji cells were permeabilized with 0.1% NP-40 in TBS for 10 minutes and blocked with 5% BSA-PBS for 30 minutes at room temperature. Raji cell were stained with ARG66996 anti-PERK antibody.
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ARG66996 anti-PERK antibody WB image
Western blot: Mouse kidney stained with ARG66996 anti-PERK antibody at 1:500 dilution by 5% SDS-PAGE.
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ARG66996 anti-PERK antibody IHC-p image
Immunohistochemistry: Formalin-fixed and paraffin-embedded human cancer tissue section tained with ARG66996 anti-PERK antibody.
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ARG66996 anti-PERK antibody WB image
Western blot: Mouse brain stained with ARG66996 anti-PERK antibody at 1:500 dilution by 5% SDS-PAGE.
