ARG58202
anti-PERK antibody
anti-PERK antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PERK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PERK |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to R222-Q334 of Human PERK. |
| 偶联标记 | Un-conjugated |
| 別名 | PRKR-like endoplasmic reticulum kinase; PERK; HsPEK; Eukaryotic translation initiation factor 2-alpha kinase 3; Pancreatic eIF2-alpha kinase; WRS; PEK; EC 2.7.11.1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 140 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2% Trehalose. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NZJ5 Human Eukaryotic translation initiation factor 2-alpha kinase 3 Swiss-port # Q9Z1Z1 Rat Eukaryotic translation initiation factor 2-alpha kinase 3 |
|---|---|
| 基因名称 | EIF2AK3 |
| 全名 | eukaryotic translation initiation factor 2-alpha kinase 3 |
| 背景介绍 | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015] |
| 生物功能 | Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity). [UniProt] |
| 预测分子量 | 125 kDa |
| 翻译后修饰 | Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity). Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. N-glycosylated. ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58202 anti-PERK antibody WB image
Western blot: 1) HeLa, 2) COLO320, 3) A549, 4) SK-OV-3, 5) A431, 6) Rat brain and 7) Mouse brain lysates stained with ARG58202 anti-PERK antibody at 0.5 µg/ml dilution.
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ARG58202 anti-PERK antibody FACS image
Flow Cytometry: HepG2 cells were blocked with goat sera and stained with ARG58202 anti-PERK antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).
