ARG57836
anti-PEG10 antibody
anti-PEG10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PEG10 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PEG10 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human PEG10. |
| 偶联标记 | Un-conjugated |
| 別名 | MEF3L; Retrotransposon-derived gag-like polyprotein; RGAG3; Paternally expressed gene 10 protein; Myelin expression factor 3-like protein 1; MEF3-like protein 1; Mart2; Ty3/Gypsy-like protein; Retrotransposon-derived protein PEG10; Embryonal carcinoma differentiation-regulated protein; EDR; Mar2; Mammalian retrotransposon-derived protein 2; Retrotransposon gag domain-containing protein 3; HB-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | SW620 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q7TN75 Mouse Retrotransposon-derived protein PEG10 Swiss-port # Q86TG7 Human Retrotransposon-derived protein PEG10 |
|---|---|
| 基因名称 | PEG10 |
| 全名 | paternally expressed 10 |
| 背景介绍 | This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene. [provided by RefSeq, Oct 2014] |
| 生物功能 | Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter (By similarity). [UniProt] |
| 细胞定位 | Nucleus, Cytoplasm. [UniProt] |
| 预测分子量 | 80 kDa |
| 翻译后修饰 | Isoform 1 undergoes proteolytic cleavage. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG57836 anti-PEG10 antibody WB image
Western blot: 25 µg of SW620 cell lysate stained with ARG57836 anti-PEG10 antibody at 1:1000 dilution.
