ARG66861
anti-PEA15 phospho (Ser104) antibody
anti-PEA15 phospho (Ser104) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PEA15 phospho (Ser104) |
|---|---|
| 反应物种 | Hu, Mk |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 特异性 | This antibody detects endogenous levels of PEA15 protein only when phosphorylated at Ser104. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PEA15 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the phosphorylated Ser104 (aa. 70-119) of Human PEA15. |
| 偶联标记 | Un-conjugated |
| 別名 | MAT1H; MAT1; Astrocytic phosphoprotein PEA-15; HUMMAT1H; 15 kDa phosphoprotein enriched in astrocytes; PED; PEA-15; Phosphoprotein enriched in diabetes; HMAT1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | COS7 | ||||||
| 实际分子量 | ~ 19 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PEA15 |
| 全名 | phosphoprotein enriched in astrocytes 15 |
| 背景介绍 | This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface. [UniProt] |
| 细胞定位 | Cytoplasm. Note=Associated with microtubules. [UniProt] |
| 预测分子量 | 15 kDa |
| 翻译后修饰 | Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66861 anti-PEA15 phospho (Ser104) antibody WB image
Western blot: COS7 cells treated with TNF at 20 ng/ml for 5 min. Cell lysates were stained with ARG66861 anti-PEA15 phospho (Ser104) antibody. The lane on the right is blocked with the phospho peptide.
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ARG66861 anti-PEA15 phospho (Ser104) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG66861 anti-PEA15 phospho (Ser104) antibody. The picture on the right is blocked with the phospho peptide.
