ARG45938

anti-PDZK1 antibody

anti-PDZK1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes PDZK1
反应物种 Ms, Rat
应用 FACS, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PDZK1
抗原物种 Mouse
抗原 Recombinant protein containing to mouse PDZK1.
偶联标记 Un-conjugated
別名 PDZK1; PDZ Domain Containing 1; CAP70; CLAMP; PDZD1; NHERF3; NHERF-3; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; NHERF-3; CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 70 kDa

属性

形式 Liquid
纯化 Affinity chromatography purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 59020 Mouse PDZK1

GeneID: 65144 Rat PDZK1

Swiss-port # Q9JIL4 Mouse Na(+)/H(+) exchange regulatory cofactor NHE-RF3

Swiss-port # Q9JJ40 Rat Na(+)/H(+) exchange regulatory cofactor NHE-RF3

基因名称 PDZK1
全名 PDZ Domain Containing 1
背景介绍 This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
生物功能 A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity). [UniProt]
细胞定位 Cell membrane; Membrane. [UniProt]
预测分子量 57 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45938 anti-PDZK1 antibody FACS image

    Flow Cytometry: RM-1 stained with ARG45938 anti-PDZK1 antibody at 1 µg/10^6 cells dilution.

  • ARG45938 anti-PDZK1 antibody WB image

    Western blot: Rat kidney stained with ARG45938 anti-PDZK1 antibody at 0.5 μg/ml dilution.

  • ARG45938 anti-PDZK1 antibody WB image

    Western blot: Mouse kidney stained with ARG45938 anti-PDZK1 antibody at 0.5 μg/ml dilution.