ARG57014
anti-PDZK1 antibody [1A2]
anti-PDZK1 antibody [1A2] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1A2] recognizes PDZK1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1A2 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | PDZK1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-519 of Human PDZK1. |
| 偶联标记 | Un-conjugated |
| 別名 | CAP70; CLAMP; PDZD1; NHERF3; NHERF-3; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; NHERF-3; CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q5T2W1 Human Na(+)/H(+) exchange regulatory cofactor NHE-RF3 |
|---|---|
| 基因名称 | PDZK1 |
| 全名 | PDZ domain containing 1 |
| 背景介绍 | This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| 生物功能 | A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity). [UniProt] |
| 预测分子量 | 57 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57014 anti-PDZK1 antibody [1A2] FACS image
Flow Cytometry: Hep3B cell line stained with ARG57014 anti-PDZK1 antibody [1A2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57014 anti-PDZK1 antibody [1A2] WB image
Western blot: 40 µg of HeLa and 293T cell lysate stained with ARG57014 anti-PDZK1 antibody [1A2] at 1:1000.
