ARG40409
anti-PDP1 antibody
anti-PDP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDP1 |
| 抗原物种 | Human |
| 抗原 | Fusion protein of Human PDP1. |
| 偶联标记 | Un-conjugated |
| 別名 | PDPC; PDPC 1; PDP; EC 3.1.3.43; Protein phosphatase 2C; PDH; PDP 1; PPM2C; [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; Pyruvate dehydrogenase phosphatase catalytic subunit 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: Human bladder carcinoma tissue. IHC-P: Human thyroid cancer and Human cervical cancer. |
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| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | 1.3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9P0J1 Human [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondria |
|---|---|
| 基因名称 | PDP1 |
| 全名 | pyruvate dehyrogenase phosphatase catalytic subunit 1 |
| 背景介绍 | Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009] |
| 生物功能 | Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. [UniProt] |
| 细胞定位 | Mitochondrion matrix. [UniProt] |
| 预测分子量 | 61 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG40409 anti-PDP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG40409 anti-PDP1 antibody (left) at 1:25 dilution, or the same antibody pre-incubated with fusion protein (right).
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ARG40409 anti-PDP1 antibody WB image
Western blot: 40 µg of Human bladder carcinoma tissue lysate stained with ARG40409 anti-PDP1 antibody at 1:300 dilution.
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ARG40409 anti-PDP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cervical cancer tissue stained with ARG40409 anti-PDP1 antibody (left) at 1:25 dilution, or the same antibody pre-incubated with fusion protein (right).
