ARG54870
anti-PDK4 antibody
anti-PDK4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDK4 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDK4 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 382-410 (C-terminus) of Human PDK4. |
| 偶联标记 | Un-conjugated |
| 別名 | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human heart |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O70571 Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochond Swiss-port # Q16654 Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochond |
|---|---|
| 基因名称 | PDK4 |
| 全名 | pyruvate dehydrogenase kinase, isozyme 4 |
| 背景介绍 | This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008] |
| 生物功能 | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism. [UniProt] |
| 细胞定位 | Mitochondrion matrix. |
| 研究领域 | Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 46 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG54870 anti-PDK4 antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then stained with ARG54870 anti-PDK4 antibody (green) at 1:100 dilution, 2 h at room temperature. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).
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ARG54870 anti-PDK4 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skeletal muscle stained with ARG54870 anti-PDK4 antibody.
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ARG54870 anti-PDK4 antibody WB image
Western blot: 20 µg of Human heart lysate stained with ARG54870 anti-PDK4 antibody at 1:2000 dilution.
