ARG40807
anti-PDK3 antibody
anti-PDK3 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDK3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDK3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 137-406 of Human PDK3 (NP_005382.1). |
| 偶联标记 | Un-conjugated |
| 別名 | CMTX6; GS1-358P8.4; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Mouse kidney | ||||
| 实际分子量 | 47 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q15120 Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochond Swiss-port # Q922H2 Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochond |
|---|---|
| 基因名称 | PDK3 |
| 全名 | pyruvate dehydrogenase kinase, isozyme 3 |
| 背景介绍 | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| 生物功能 | Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species. [UniProt] |
| 细胞定位 | Mitochondrion matrix. [UniProt] |
| 预测分子量 | 47 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG40807 anti-PDK3 antibody WB image
Western blot: 25 µg of Mouse kidney lysate stained with ARG40807 anti-PDK3 antibody at 1:1000 dilution.
