ARG55843
anti-PDIA6 antibody
anti-PDIA6 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDIA6 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDIA6 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 144-172 (Center) of Human PDIA6. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein disulfide isomerase P5; ERP5; Thioredoxin domain-containing protein 7; P5; ER protein 5; Endoplasmic reticulum protein 5; ERp5; EC 5.3.4.1; Protein disulfide-isomerase A6; TXNDC7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDIA6 |
| 全名 | protein disulfide isomerase family A, member 6 |
| 背景介绍 | Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM, Mar 2008] |
| 生物功能 | May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. [UniProt] |
| 细胞定位 | Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138}. Cell membrane. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| 预测分子量 | 48 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG55843 anti-PDIA6 antibody ICC/IF image
Immunofluorescence: HepG2 cells stained with ARG55843 anti-PDIA6 antibody (green) at 1:100 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). DAPI (blue) for nuclear staining.
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ARG55843 anti-PDIA6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG55843 anti-PDIA6 antibody at 1:100 dilution.
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ARG55843 anti-PDIA6 antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG55843 anti-PDIA6 antibody at 1:1000 dilution.
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ARG55843 anti-PDIA6 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG55843 anti-PDIA6 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
