ARG22213

anti-PDIA3 / ERp57 antibody [MaP.Erp57]

anti-PDIA3 / ERp57 antibody [MaP.Erp57] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Dog,Guinea pig,Hamster,Monkey,Pig,Rabbit

概述

产品描述 Mouse Monoclonal antibody [Map.ERP57] recognizes PDIA3 / ERp57
反应物种 Hu, Ms, Rat, Bov, Dog, Gpig, Hm, Mk, Pig, Rb
应用 ICC/IF, IHC-P, IP, WB
特异性 Detects ~57kDa.
宿主 Mouse
克隆 Monoclonal
克隆号 Map.ERP57
同位型 IgG1
靶点名称 PDIA3 / ERp57
抗原物种 Human
抗原 Human recombinant ERp57
偶联标记 Un-conjugated
別名 EC 5.3.4.1; Disulfide isomerase ER-60; HEL-S-93n; GRP57; p58; Endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; ERp57; GRP58; P58; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER60; HEL-S-269; Protein disulfide-isomerase A3; PI-PLC; ERp60; ERp61; HsT17083; Endoplasmic reticulum resident protein 57

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100
IHC-P1:100
IPAssay-dependent
WB1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂 0.09% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14827 Mouse PDIA3

GeneID: 2923 Human PDIA3

GeneID: 29468 Rat PDIA3

基因名称 PDIA3
全名 protein disulfide isomerase family A, member 3
背景介绍 This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
细胞定位 Endoplasmic Reticulum, Endoplasmic reticulum lumen, Melanosome
产品亮点 Related products:
ERp57 antibodies; Anti-Mouse IgG secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
预测分子量 57 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] WB image

    Western blot: Human cell lysates stained with ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] at 1:1000 dilution.