ARG42504
anti-PDIA3 / ERp57 antibody
anti-PDIA3 / ERp57 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Monkey
概述
| 产品描述 | Goat Polyclonal antibody recognizes PDIA3 / ERp57 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDIA3 / ERp57 |
| 抗原物种 | Human |
| 抗原 | Recombinante peptide within aa. 300 to the C-terminus of Human PDIA3 / ERp57. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 5.3.4.1; Disulfide isomerase ER-60; HEL-S-93n; GRP57; p58; Endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; ERp57; GRP58; P58; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER60; HEL-S-269; Protein disulfide-isomerase A3; PI-PLC; ERp60; ERp61; HsT17083; Endoplasmic reticulum resident protein 57 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Hepa and TR-iBRB | ||||||||||
| 实际分子量 | ~ 57 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDIA3 |
| 全名 | protein disulfide isomerase family A, member 3 |
| 背景介绍 | This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016] |
| 细胞定位 | Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). [UniProt] |
| 预测分子量 | 57 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42504 anti-PDIA3 / ERp57 antibody ICC/IF image
Immunofluorescence: Primary RPE cells were fixed with 4% PFA. Cells were stained with ARG42504 anti-PDIA3 / ERp57 antibody (green) at 1:100 dilution. Nuclear staining (blue).
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ARG42504 anti-PDIA3 / ERp57 antibody WB image
Western blot: 100 µg of Hepa and TR-iBRB cell lysates stained with ARG42504 anti-PDIA3 / ERp57 antibody at 1:500 dilution.
