ARG63726
anti-PDE5A antibody
anti-PDE5A antibody for Flow cytometry,ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PDE5A |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Rat, Dog |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDE5A |
| 抗原物种 | Human |
| 抗原 | C-LINGESGQAKRN |
| 偶联标记 | Un-conjugated |
| 別名 | cGMP-specific 3',5'-cyclic phosphodiesterase; CN5A; CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; PDE5; EC 3.1.4.35 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O76074 Human cGMP-specific 3',5'-cyclic phosphodiesterase |
|---|---|
| 背景介绍 | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation. |
检测图片 (4) Click the Picture to Zoom In
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ARG63726 anti-PDE5A antibody WB image
Western Blot: human lung lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63726 anti-PDE5A antibody at 0.3 µg/ml dilution.
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ARG63726 anti-PDE5A antibody WB image
Western Blot: 1). Mock transfection; 2) PDE5A (RC218974) expressing plasmid transfected HEK293 cell lysate standed with ARG63726 anti-PDE5A antibody
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ARG63726 anti-PDE5A antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63726 anti-PDE5A antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63726 anti-PDE5A antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63726 anti-PDE5A antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
