ARG10805
anti-PDE5A antibody
anti-PDE5A antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Pig
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDE5A |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 应用 | Confocal, Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDE5A |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide taken within aa. 120-170 from Human PDE5A. |
| 偶联标记 | Un-conjugated |
| 別名 | cGMP-specific 3',5'-cyclic phosphodiesterase; CN5A; CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; PDE5; EC 3.1.4.35 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 30% Glycerol and 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDE5A |
| 全名 | phosphodiesterase 5A, cGMP-specific |
| 背景介绍 | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP. [UniProt] |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation. |
检测图片 (2) Click the Picture to Zoom In
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ARG10805 anti-PDE5A antibody WB image
Western blot: Human PDE5A-GFP overexpressing cells untreated or treated with ANP. The blots were stained with ARG10805 anti-PDE5A antibody at 1:500 dilution.
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ARG10805 anti-PDE5A antibody Confocal image
Confocal microscopy: Cells expressing PDE5A stained with ARG10805 anti-PDE5A antibody at 1:200 dilution.
