ARG10819
anti-PDE4D phospho (Ser190) antibody
anti-PDE4D phospho (Ser190) antibody for Dot blot,ELISA,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDE4D phospho (Ser190) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | Dot, ELISA, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDE4D |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide selective for PDE4D PKA site taken within aa. 50-100 from Human PDE4D. Derived around the PKA phosphorylation site of Ser190: R-E-Sp-F-L. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.1.4.53; STRK1; DPDE3; PDE43; ACRDYS2; PDE4DN2; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; HSPDE4D |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 30% Glycerol and 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDE4D |
| 全名 | phosphodiesterase 4D, cAMP-specific |
| 背景介绍 | This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009] |
| 生物功能 | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [UniProt] |
| 预测分子量 | 91 kDa |
| 翻译后修饰 | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
检测图片 (1) Click the Picture to Zoom In
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ARG10819 anti-PDE4D phospho (Ser190) antibody WB image
Western blot: Non-phosphorylated and phosphorylated PDE4D protein stained with ARG10819 anti-PDE4D phospho (Ser190) antibody and ARG10803 anti-PDE4D antibody at 1:500 dilution.
