ARG10799
anti-PDE4 antibody
anti-PDE4 antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Chicken,Monkey
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDE4 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Chk, Mk |
| 应用 | Confocal, Dot, ELISA, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDE4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide with a sequence common to most PDE4 subtypes: PDE4A, PDE4D, PDE4B. |
| 偶联标记 | Un-conjugated |
| 別名 | DPDE2; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; PDE4; PDE46; EC 3.1.4.53 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 30% Glycerol and 0.5% BSA |
| 浓度 | 0.62 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDE4A |
| 全名 | phosphodiesterase 4A, cAMP-specific |
| 背景介绍 | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| 生物功能 | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [UniProt] |
| 预测分子量 | 98 kDa |
| 翻译后修饰 | Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation (By similarity). Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA. Proteolytically cleaved by caspase-3. |
检测图片 (1) Click the Picture to Zoom In
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ARG10799 anti-PDE4 antibody WB image
Western blot: Rat cortical membranes stained with ARG10799 anti-PDE4 antibody at 1:500 dilution.
