ARG56950
anti-PDCD4 antibody [k4C1]
anti-PDCD4 antibody [k4C1] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [k4C1] recognizes PDCD4 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | k4C1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | PDCD4 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-469 of Human PDCD4. |
| 偶联标记 | Un-conjugated |
| 別名 | Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; Programmed cell death protein 4; H731 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDCD4 |
| 全名 | programmed cell death 4 (neoplastic transformation inhibitor) |
| 背景介绍 | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] |
| 生物功能 | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity). [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4. |
检测图片 (1) Click the Picture to Zoom In
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ARG56950 anti-PDCD4 antibody [k4C1] WB image
Western blot: 40 µg of HepG2 cell lysate stained with ARG56950 anti-PDCD4 antibody [k4C1] at 1:2000.
