ARG42238
anti-PDCD4 antibody
anti-PDCD4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDCD4 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDCD4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human PDCD4. |
| 偶联标记 | Un-conjugated |
| 別名 | Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; Programmed cell death protein 4; H731 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | HeLa | ||||||||||
| 实际分子量 | ~ 52 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDCD4 |
| 全名 | programmed cell death 4 (neoplastic transformation inhibitor) |
| 背景介绍 | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] |
| 生物功能 | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133). [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42238 anti-PDCD4 antibody WB image
Western blot: MCF-7 stained with ARG42238 anti-PDCD4 antibody.
From Chiglintseva D et al. Biomaterials. (2024), doi: 10.1016/j.biomaterials.2024.122604, Fig. 6. C.
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ARG42238 anti-PDCD4 antibody WB image
Western blot: HeLa cell lysate stained with ARG42238 anti-PDCD4 antibody.
文献引用
Engineering supramolecular dynamics of self-assembly and turnover of oncogenic microRNAs to drive their synergistic destruction in tumor models
WB / Human
