ARG42238

anti-PDCD4 antibody

anti-PDCD4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes PDCD4
反应物种 Hu, Ms
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PDCD4
抗原物种 Human
抗原 Synthetic peptide of Human PDCD4.
偶联标记 Un-conjugated
別名 Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; Programmed cell death protein 4; H731

应用说明

应用建议
应用 推荐稀释比
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:100 - 1:500
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa
实际分子量 ~ 52 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 18569 Mouse PDCD4

GeneID: 27250 Human PDCD4

Swiss-port # Q53EL6 Human Programmed cell death protein 4

Swiss-port # Q61823 Mouse Programmed cell death protein 4

基因名称 PDCD4
全名 programmed cell death 4 (neoplastic transformation inhibitor)
背景介绍 This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
生物功能 Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity). [UniProt]
细胞定位 Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133). [UniProt]
预测分子量 52 kDa
翻译后修饰 Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG42238 anti-PDCD4 antibody WB image

    Western blot: MCF-7 stained with ARG42238 anti-PDCD4 antibody.

    From Chiglintseva D et al. Biomaterials. (2024), doi: 10.1016/j.biomaterials.2024.122604, Fig. 6. C.

  • ARG42238 anti-PDCD4 antibody WB image

    Western blot: HeLa cell lysate stained with ARG42238 anti-PDCD4 antibody.

文献引用

Engineering supramolecular dynamics of self-assembly and turnover of oncogenic microRNAs to drive their synergistic destruction in tumor models

WB / Human

Daria Chiglintseva et al.
Biomaterials.,  (2024)

publication_link

 

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