ARG62605
anti-PCNA antibody [PC10]
anti-PCNA antibody [PC10] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Chicken,D. melanogaster ,Human,Monkey,Mouse,Pig,Pigeon,Rat,Zebrafish
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
参考文献9
概述
| 产品描述 | Mouse Monoclonal antibody [PC10] recognizes PCNA |
|---|---|
| 反应物种 | Hu, Ms, Rat, Chk, Dm, Mk, Pgn, Pig, Zfsh |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PC10 |
| 同位型 | IgG2a |
| 靶点名称 | PCNA |
| 抗原物种 | Rat |
| 抗原 | Protein A-rat PCNA (proliferating cell nuclear antigen) fusion obtained from pC2T |
| 偶联标记 | Un-conjugated |
| 別名 | PCNA; ATLD2; Cyclin; Proliferating cell nuclear antigen |
应用说明
| 应用建议 |
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | ICC/IF: Methanol fixed samples. IHC-P: Antigen Retrieval: Heat mediated in Citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||||
| 阳性对照 | HEK293 |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS (pH 7.6), 0.02% Sodium azide and 0.4M arginine. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Pcna |
| 全名 | proliferating cell nuclear antigen |
| 背景介绍 | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. [uniprot: P12004] |
| 产品亮点 | Related products: PCNA antibodies; PCNA Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956). Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902). Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956). Ubiquitinated (PubMed:24939902, PubMed:20227374). Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. Methylated on glutamate residues by ARMT1/C6orf211. |
检测图片 (8) Click the Picture to Zoom In
-
ARG62605 anti-PCNA antibody [PC10] IHC-P image
Immunohistochemistry: Mouse xenograft tumor stained with ARG22104 anti-Ly6G + Ly6C antibody [RB6-8C5] ARG40666 anti-mTOR phospho (Ser2448) antibody , ARG51558 anti-Akt phospho (Ser473) antibody and ARG62605 anti-PCNA antibody [PC10].
From Wu LH et al. J Cancer (2022), doi: 10.7150/jca.75163, Fig. 5. D.
-
ARG62605 anti-PCNA antibody [PC10] IHC-P image
Immunohistochemistry: HSC6 stained with ARG62605 anti-PCNA antibody [PC10].
From Hu Q et al. Cell Death Dis- (2020), doi: 10.1038/s41419-020-03126-0, Fig. 1. K.
-
ARG62605 anti-PCNA antibody [PC10] IHC-P image
Immunohistochemistry: Mouse tibia growth plate stained with ARG62605 anti-PCNA antibody [PC10].
From Chou LY et al. FASEB J- (2020), doi: 10.1096/fj.201901852RR, Fig. 3. E.
-
ARG62605 anti-PCNA antibody [PC10] IHC-P image
Immunohistochemistry: Porcine kidney stained with ARG62605 anti-PCNA antibody [PC10].
From Huang J et al. Frontiers in Cell and Developmental Biology (2022), doi: 10.3389/fcell.2022.899869, Fig. 6. A.
-
ARG62605 anti-PCNA antibody [PC10] WB image
Western blot: Y79 stained with ARG62605 anti-PCNA antibody [PC10].
From Chen M et al. Cell Death Dis (2022), doi: 10.1038/s41419-022-05222-9, Fig. 2. J.
-
ARG62605 anti-PCNA antibody [PC10] WB image
Western blot: Porcine kidney stained with ARG62605 anti-PCNA antibody [PC10].
From Huang J et al. Frontiers in Cell and Developmental Biology (2022), doi: 10.3389/fcell.2022.899869, Fig. 6. C.
-
ARG62605 anti-PCNA antibody [PC10] WB image
Western blot: A549, H1299, HCT116 and HepG2 cell lysates stained with ARG62605 anti-PCNA antibody [PC10] at 0.2 µg/ml.
-
ARG62605 anti-PCNA antibody [PC10] IHC-P image
Immunohistochemistry: formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with PCNA antibody [PC10] (ARG62605).
客户反馈
Excellent
anti-PCNA antibody [PC10]
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Downregulation of AKT/mTOR signaling pathway for Salmonella-mediated autophagy in human anaplastic thyroid cancer
IHC-P / Mouse
Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model
WB / Pig
E2F1/CKS2/PTEN signaling axis regulates malignant phenotypes in pediatric retinoblastoma
WB / Human
Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.
IHC-P / Mouse
Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.
IHC-P / Human
Discoidin domain receptor 1 regulates endochondral ossification through terminal differentiation of chondrocytes.
IHC-P / Mouse
Protein arginine methyltransferase 1 mediates renal fibroblast activation and fibrogenesis through activation of Smad3 signaling.
IHC-P / Rat
CSN6 expression is associated with pancreatic cancer progression and predicts poor prognosis.
WB, IHC-P / Human
STAT1-mediated inhibition of FOXM1 enhances gemcitabine sensitivity in pancreatic cancer.
IHC-P / Human
