ARG40108
anti-PCM1 antibody
anti-PCM1 antibody for ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PCM1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PCM1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-260 of Human PCM1 (NP_006188.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Pericentriolar material 1 protein; RET/PCM-1; hPCM-1; PTC4; PCM-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Raji | ||||||
| 实际分子量 | 280 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PCM1 |
| 全名 | pericentriolar material 1 |
| 背景介绍 | The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. [provided by RefSeq, Sep 2010] |
| 生物功能 | Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body. Note=Recruitment to the centrosome requires microtubules and dynein. [UniProt] |
| 预测分子量 | 229 kDa |
| 翻译后修饰 | Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40108 anti-PCM1 antibody WB image
Western blot: 25 µg of Raji cell lysate stained with ARG40108 anti-PCM1 antibody at 1:1000 dilution.
