ARG66582
anti-PCCB antibody
anti-PCCB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PCCB |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PCCB |
| 抗原物种 | Human |
| 抗原 | Recombinant full length protein of Human PCCB. |
| 偶联标记 | Un-conjugated |
| 別名 | PCCase subunit beta; EC 6.4.1.3; Propanoyl-CoA:carbon dioxide ligase subunit beta; Propionyl-CoA carboxylase beta chain, mitochondrial |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P05166 Human Propionyl-CoA carboxylase beta chain, mitochondrial Swiss-port # Q99MN9 Mouse Propionyl-CoA carboxylase beta chain, mitochondrial |
|---|---|
| 基因名称 | PCCB |
| 全名 | propionyl CoA carboxylase, beta polypeptide |
| 背景介绍 | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
| 细胞定位 | Mitochondrion matrix. [UniProt] |
| 预测分子量 | 58 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG66582 anti-PCCB antibody ICC/IF image
Immunofluorescence: Formalin-fixed U2OS cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66582 anti-PCCB antibody (red) in 3% BSA-PBS and incubated overnight at 4°C.
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ARG66582 anti-PCCB antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66582 anti-PCCB antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.
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ARG66582 anti-PCCB antibody WB image
Western blot: HT29, Mouse liver and Mouse heart lysates stained with ARG66582 anti-PCCB antibody.
