ARG40796
anti-PCBP2 / hnRNP E2 antibody
anti-PCBP2 / hnRNP E2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PCBP2 / hnRNP E2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PCBP2 / hnRNP E2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-130 of Human PCBP2 (NP_001122383.1). |
| 偶联标记 | Un-conjugated |
| 別名 | HNRPE2; HNRNPE2; hnRNP-E2; Poly(rC)-binding protein 2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2; hnRNP E2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse spleen | ||||||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PCBP2 |
| 全名 | poly(rC) binding protein 2 |
| 背景介绍 | The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. [UniProt] |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. (Microbial infection) Proteolyticaly cleaved by picornavirus proteinase 3CD. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40796 anti-PCBP2 / hnRNP E2 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG40796 anti-PCBP2 / hnRNP E2 antibody.
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ARG40796 anti-PCBP2 / hnRNP E2 antibody WB image
Western blot: 25 µg of Mouse spleen lysate stained with ARG40796 anti-PCBP2 / hnRNP E2 antibody at 1:1000 dilution.
