ARG45409

anti-PCBP1 antibody

anti-PCBP1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes PCBP1
反应物种 Hu, Ms, Rat
应用 FACS, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PCBP1
抗原物种 Human
抗原 Recombinant protein containing to human PCBP1.
偶联标记 Un-conjugated
別名 HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; Poly(rC)-binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 40 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.005% Sodium azide and 4% Trehalose.
抗菌剂 0.005% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 23983 Mouse PCBP1

GeneID: 5093 Human PCBP1

Swiss-port # P60335 Mouse Poly(rC)-binding protein 1

Swiss-port # Q15365 Human Poly(rC)-binding protein 1

基因名称 PCBP1
全名 poly(rC) binding protein 1
背景介绍 This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
生物功能 Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. [UniProt]
细胞定位 Nucleus. Cytoplasm. [UniProt]
预测分子量 37 kDa
翻译后修饰 Phosphorylated; lowers poly(rC)-binding activity. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45409 anti-PCBP1 antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG45409 anti-PCBP1 antibody at 2 μg/ml dilution.

  • ARG45409 anti-PCBP1 antibody WB image

    Western blot: HepG2 stained with ARG45409 anti-PCBP1 antibody at 0.5 μg/ml dilution.

  • ARG45409 anti-PCBP1 antibody FACS image

    Flow Cytometry: CACO-2 stained with ARG45409 anti-PCBP1 antibody at 1 µg/10^6 cells dilution.

  • ARG45409 anti-PCBP1 antibody WB image

    Western blot: NIH/3T3 stained with ARG45409 anti-PCBP1 antibody at 0.5 μg/ml dilution.