ARG66821
anti-PAX8 antibody [SQab20233]
anti-PAX8 antibody [SQab20233] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20233] recognizes PAX8 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20233 |
| 同位型 | IgG |
| 靶点名称 | PAX8 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human PAX8. |
| 偶联标记 | Un-conjugated |
| 別名 | Paired box protein Pax-8 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PAX8 |
| 全名 | paired box 8 |
| 背景介绍 | This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010] |
| 生物功能 | Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 48 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG66821 anti-PAX8 antibody [SQab20233] WB image (Customer's Feedback)
Western blot: MCF7 cell lysate stained with ARG66821 anti-PAX8 antibody [SQab20233] at 1:1000 dilution.
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ARG66821 anti-PAX8 antibody [SQab20233] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thyroid cancer tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66821 anti-PAX8 antibody [SQab20233].
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ARG66821 anti-PAX8 antibody [SQab20233] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human serous cystadenocarcinoma of the ovary tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66821 anti-PAX8 antibody [SQab20233].
客户反馈
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anti-PAX8 antibody [SQab20233]
Application：WB
Sample：MCF7
Sample Loading Amount：20 μg
SDS Page Type：Denatured
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
