ARG59986
anti-PASK antibody
anti-PASK antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PASK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PASK |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1194-1323 of Human PASK (NP_055963.2). |
| 偶联标记 | Un-conjugated |
| 別名 | PAS domain-containing serine/threonine-protein kinase; hPASK; PASKIN; STK37; PAS-kinase; EC 2.7.11.1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | 190 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8CEE6 Mouse PAS domain-containing serine/threonine-protein kinase Swiss-port # Q96RG2 Human PAS domain-containing serine/threonine-protein kinase |
|---|---|
| 基因名称 | PASK |
| 全名 | PAS domain containing serine/threonine kinase |
| 背景介绍 | This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| 生物功能 | Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails. [UniProt] |
| 预测分子量 | 143 kDa |
| 翻译后修饰 | Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59986 anti-PASK antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG59986 anti-PASK antibody at 1:1000 dilution.
