ARG63398
anti-PARD6A / PAR6 antibody
anti-PARD6A / PAR6 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PARD6A / PAR6 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PARD6A / PAR6 |
| 抗原物种 | Human |
| 抗原 | C-GSRIRGDGSGFSL |
| 偶联标记 | Un-conjugated |
| 別名 | Tax interaction protein 40; TIP-40; PAR-6 alpha; PAR6; PAR6alpha; PAR-6A; PAR-6; PAR6C; Partitioning defective 6 homolog alpha; TAX40 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NPB6 Human Partitioning defective 6 homolog alpha |
|---|---|
| 背景介绍 | This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Phosphorylated by the TGF-beta receptor. |
检测图片 (4) Click the Picture to Zoom In
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ARG63398 anti-PARD6A / PAR6 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Pancreas. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63398 anti-PARD6A / PAR6 antibody at 10 µg/ml dilution followed by HRP-staining.
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ARG63398 anti-PARD6A / PAR6 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63398 anti-PARD6A / PAR6 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63398 anti-PARD6A / PAR6 antibody WB image
Western blot: 35 µg of Jurkat (A) and U251 (B) cell lysates (in RIPA buffer) stained with ARG63398 anti-PARD6A / PAR6 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
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ARG63398 anti-PARD6A / PAR6 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton. Cells were stained with ARG63398 anti-PARD6A / PAR6 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
