ARG66841
anti-PAK5 / PAK7 antibody
anti-PAK5 / PAK7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PAK5 / PAK7 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PAK5 / PAK7 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide between aa. 661-710 of Human PAK7. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.11.1; Serine/threonine-protein kinase PAK 7; PAK5; p21-activated kinase 7; p21-activated kinase 5; PAK-7; PAK-5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human brain and NIH/3T3 | ||||||
| 实际分子量 | 80 ~ 90 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8C015 Mouse Serine/threonine-protein kinase PAK 7 Swiss-port # Q9P286 Human Serine/threonine-protein kinase PAK 7 |
|---|---|
| 基因名称 | PAK7 |
| 全名 | p21 protein (Cdc42/Rac)-activated kinase 7 |
| 背景介绍 | The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions. [UniProt] |
| 细胞定位 | Mitochondrion. Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival. [UniProt] |
| 预测分子量 | 81 kDa |
| 翻译后修饰 | Autophosphorylated when activated by CDC42/p21. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG66841 anti-PAK5 / PAK7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66841 anti-PAK5 / PAK7 antibody. The lane on the right is blocked with the peptide.
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ARG66841 anti-PAK5 / PAK7 antibody WB image
Western blot: NIH/3T3 cell lysate stained with ARG66841 anti-PAK5 / PAK7 antibody.
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ARG66841 anti-PAK5 / PAK7 antibody WB image
Western blot: Human brain lysates stained with ARG66841 anti-PAK5 / PAK7 antibody. The lane on the right is blocked with the peptide.
