ARG64016
anti-PAK1 antibody
anti-PAK1 antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PAK1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Dog |
| 应用 | WB |
| 特异性 | No cross-reactivity exptected with PAK3 |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PAK1 |
| 抗原物种 | Human |
| 抗原 | C-NTEKQKKKPKMSDE |
| 偶联标记 | Un-conjugated |
| 別名 | PAKalpha; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p65-PAK; EC 2.7.11.1; PAK-1; p21-activated kinase 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P35465 Rat Serine/threonine-protein kinase PAK 1 Swiss-port # Q13153 Human Serine/threonine-protein kinase PAK 1 |
|---|---|
| 背景介绍 | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites. |
检测图片 (2) Click the Picture to Zoom In
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ARG64016 anti-PAK1 antibody WB image
Western Blot: Rat Brain lysate (35 µg protein in RIPA buffer) stained with ARG64016 anti-PAK1 antibody at 1 µg/ml dilution.
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ARG64016 anti-PAK1 antibody WB image
Western blot: 35 µg of Human brain (cerebral cortex) lysate (in RIPA buffer) stained with ARG64016 anti-PAK1 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
