ARG63083
anti-PAG / Cbp antibody [PAG-C1]
anti-PAG / Cbp antibody [PAG-C1] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [PAG-C1] recognizes PAG / Cbp |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov |
| 应用 | IHC-P, IP, WB |
| 特异性 | The clone PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PAG-C1 |
| 同位型 | IgG2b |
| 靶点名称 | PAG / Cbp |
| 抗原物种 | Human |
| 抗原 | C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. |
| 偶联标记 | Un-conjugated |
| 別名 | Transmembrane phosphoprotein Cbp; Csk-binding protein; CBP; PAG; Transmembrane adapter protein PAG; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Heat-mediated antigen retrieval in citrate buffer pH 6.1 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||
| 阳性对照 | IP: Raji IHC-P: Appendix (germinal center of lymphatic follicle) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PAG1 |
| 全名 | phosphoprotein membrane anchor with glycosphingolipid microdomains 1 |
| 背景介绍 | PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. |
| 生物功能 | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Palmitoylated. Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN. |
检测图片 (2) Click the Picture to Zoom In
-
ARG63083 anti-PAG / Cbp antibody [PAG-C1] IP image
Immunoprecipitation: Raji lysate was immunoprecipitated with ARG63083 anti-PAG / Cbp antibody [PAG-C1] (Lane 1, 2) or anti-PAG polyclonal antibody (Lane 3). Western blot was immunostained with ARG63084 anti-PAG / Cbp antibody [MEM-255].
-
ARG63083 anti-PAG / Cbp antibody [PAG-C1] IHC-P image
Immunohistochemistry: Appendix tissue section stained with ARG63083 anti-PAG / Cbp antibody [PAG-C1].
克隆号文献
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling.
Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes.
