ARG63613
anti-PACSIN1 antibody
anti-PACSIN1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PACSIN1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants represent identical protein (NP_065855.1; NP_001186512.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PACSIN1 |
| 抗原物种 | Human |
| 抗原 | SSSYDEASLAPEET-C |
| 偶联标记 | Un-conjugated |
| 別名 | SDPI; Protein kinase C and casein kinase substrate in neurons protein 1; Syndapin-1 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BY11 Human Protein kinase C and casein kinase substrate in neurons protein 1 |
|---|---|
| 基因名称 | PACSIN1 |
| 全名 | protein kinase C and casein kinase substrate in neurons 1 |
| 生物功能 | Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity). Binds to membranes via its F-BAR domain and mediates membrane tubulation. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). |
检测图片 (3) Click the Picture to Zoom In
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ARG63613 anti-PACSIN1 antibody WB image
Western Blot: Human Brain (hippocampus) lysate (35 µg protein in RIPA buffer) stained with ARG63613 anti-PACSIN1 antibody at 1 µg/ml dilution.
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ARG63613 anti-PACSIN1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63613 anti-PACSIN1 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63613 anti-PACSIN1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63613 anti-PACSIN1 antibody at 2.5 µg/ml dilution followed by AP-staining.
