概述

产品描述	Rabbit Polyclonal antibody recognizes P4HB
反应物种	Hu, Ms, Rat
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	P4HB
抗原物种	Human
抗原	Synthetic peptide derived from Human P4HB.
偶联标记	Un-conjugated
別名	Cellular thyroid hormone-binding protein; CLCRP1; PHDB; GIT; p55; P4Hbeta; PDIA1; ERBA2L; PROHB; PDI; DSI; Protein disulfide-isomerase; PO4DB; EC 5.3.4.1; Prolyl 4-hydroxylase subunit beta; PO4HB

应用说明

应用建议	应用 推荐稀释比 FACS 1:50 ICC/IF 1:50 - 1:200 IHC-P 1:100 - 1:500 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	HepG2
实际分子量	~ 53 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 18453 Mouse P4HB GeneID: 25506 Rat P4HB GeneID: 5034 Human P4HB Swiss-port # P04785 Rat Protein disulfide-isomerase Swiss-port # P07237 Human Protein disulfide-isomerase Swiss-port # P09103 Mouse Protein disulfide-isomerase More
基因名称	P4HB
全名	prolyl 4-hydroxylase, beta polypeptide
背景介绍	This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]
生物功能	This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). [UniProt]
细胞定位	ER, ER lumen. Melanosome. Cell membrane; Peripheral membrane protein. Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalizes with MTTP in the ER. [UniProt]
预测分子量	57 kDa

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