ARG59851
anti-P4HA3 antibody
anti-P4HA3 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes P4HA3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | P4HA3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 20-110 of Human P4HA3 (NP_878907.1). |
| 偶联标记 | Un-conjugated |
| 別名 | 4-PH alpha-3; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Prolyl 4-hydroxylase subunit alpha-3; EC 1.14.11.2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293T | ||||||
| 实际分子量 | 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6W3E9 Rat Prolyl 4-hydroxylase subunit alpha-3 Swiss-port # Q7Z4N8 Human Prolyl 4-hydroxylase subunit alpha-3 |
|---|---|
| 基因名称 | P4HA3 |
| 全名 | prolyl 4-hydroxylase, alpha polypeptide III |
| 背景介绍 | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. [UniProt] |
| 细胞定位 | Endoplasmic reticulum lumen. [UniProt] |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | N-glycosylation plays no role in the catalytic activity. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59851 anti-P4HA3 antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG59851 anti-P4HA3 antibody at 1:3000 dilution.
