ARG59783
anti-P4HA2 antibody
anti-P4HA2 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes P4HA2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody might cross-react to P4HA1 based on sequence analysis (63%). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | P4HA2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 234-533 of Human P4HA2 (NP_001136070.1). |
| 偶联标记 | Un-conjugated |
| 別名 | 4-PH alpha-2; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Prolyl 4-hydroxylase subunit alpha-2; EC 1.14.11.2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Rat brain, Mouse brain and SKOV3 | ||||||
| 实际分子量 | 65 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O15460 Human Prolyl 4-hydroxylase subunit alpha-2 Swiss-port # Q60716 Mouse Prolyl 4-hydroxylase subunit alpha-2 |
|---|---|
| 基因名称 | P4HA2 |
| 全名 | prolyl 4-hydroxylase, alpha polypeptide II |
| 背景介绍 | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. [UniProt] |
| 细胞定位 | Endoplasmic reticulum lumen. [UniProt] |
| 预测分子量 | 61 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG59783 anti-P4HA2 antibody WB image
Western blot: 25 µg of Rat brain, Mouse brain and SKOV3 cell lysates stained with ARG59783 anti-P4HA2 antibody at 1:1000 dilution.
