ARG43366
anti-P2RX7 / P2X7 antibody
anti-P2RX7 / P2X7 antibody for Western blot and Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes P2RX7 / P2X7 |
|---|---|
| 反应物种 | Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | P2RX7 / P2X7 |
| 抗原物种 | Mouse |
| 抗原 | Recombinant protein corresponding to N380-E585 of Mouse P2RX7 / P2X7. |
| 偶联标记 | Un-conjugated |
| 別名 | ATP receptor; P2X7; P2X purinoceptor 7; P2Z receptor; Purinergic receptor |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Neuro-2a | ||||
| 实际分子量 | ~ 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | P2RX7 |
| 全名 | purinergic receptor P2X, ligand gated ion channel, 7 |
| 背景介绍 | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010] |
| 生物功能 | Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206). [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG43366 anti-P2RX7 / P2X7 antibody WB image
Western blot: 30 µg of sample under reducing conditions. Neuro-2a whole cell lysate stained with ARG43366 anti-P2RX7 / P2X7 antibody at 0.5 µg/ml dilution, overnight at 4°C.
