ARG22576
anti-P2RX7 / P2X7 Receptor antibody [Hano43]
anti-P2RX7 / P2X7 Receptor antibody [Hano43] for Flow cytometry,ICC/IF and Mouse
概述
| 产品描述 | Rat Monoclonal antibody [Hano43] recognizes P2RX7 / P2X7 Receptor |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, ICC/IF |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | Hano43 |
| 同位型 | IgG2b |
| 靶点名称 | P2RX7 / P2X7 Receptor |
| 抗原物种 | Mouse |
| 抗原 | A P2X7-expression construct and a final boost with P2X7-transfected HEK cells. |
| 偶联标记 | Un-conjugated |
| 別名 | ATP receptor; P2X7; P2X purinoceptor 7; P2Z receptor; Purinergic receptor |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | P2rx7 |
| 全名 | purinergic receptor P2X, ligand-gated ion channel, 7 |
| 背景介绍 | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010] |
| 生物功能 | Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. [UniProt] |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. |
