ARG59235
anti-Otoferlin antibody [13A9]
anti-Otoferlin antibody [13A9] for ICC/IF and Human
概述
| 产品描述 | Mouse Monoclonal antibody [13A9] recognizes Otoferlin |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 13A9 |
| 同位型 | IgG1 |
| 靶点名称 | Otoferlin |
| 抗原物种 | Human |
| 抗原 | GST fusion protein corresponding to aa. 1-395 of Human Otoferlin (Swiss-Prot: Q9HC10). |
| 偶联标记 | Un-conjugated |
| 別名 | FER1L2; DFNB6; AUNB1; DFNB9; Fer-1-like protein 2; NSRD9; Otoferlin |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~ 220 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | 0.1M Tris, 0.1M Glycine and 2% Sucrose. |
| 稳定剂 | 2% Sucrose |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | OTOF |
| 全名 | otoferlin |
| 背景介绍 | Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes (By similarity). [UniProt] |
| 细胞定位 | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. [UniProt] |
| 预测分子量 | 227 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG59235 anti-Otoferlin antibody [13A9] ICC/IF image
Immunofluorescence: Hair cells of the inner ear crista ampullaris (CA), utricular macula (UM) and saccularmacula (SM) stained with ARG59235 anti-Otoferlin antibody [13A9].
