ARG56173
anti-Ornithine Decarboxylase antibody [ODC1/485]
anti-Ornithine Decarboxylase antibody [ODC1/485] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [ODC1/485] recognizes Ornithine Decarboxylase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ODC1/485 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Ornithine Decarboxylase |
| 抗原物种 | Human |
| 抗原 | Recombinant human Ornithine Decarboxylase. |
| 偶联标记 | Un-conjugated |
| 別名 | Ornithine decarboxylase; ODC; EC 4.1.1.17 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | PC3 | ||||||||||
| 实际分子量 | ~ 48 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ODC1 |
| 全名 | ornithine decarboxylase 1 |
| 背景介绍 | This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2013] |
| 生物功能 | Key enzyme of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues. |
检测图片 (8) Click the Picture to Zoom In
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] ICC/IF image
Immunofluorescence: LNCaP cells stained with Alexa Fluor 488-conjugated ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] FACS image
Flow Cytometry: permeablized PC3 cells stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate carcinoma stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse pancreas tissue stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] WB image
Western blot: Human Placenta stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] WB image
Western blot: PC3 cell lysate stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat pancreas stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
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ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testicular carcinoma stained with ARG56173 anti-Ornithine Decarboxylase antibody [ODC1/485].
