anti-Occludin antibody

anti-Occludin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Signaling Transduction antibody
publication_link 参考文献3



Rabbit Polyclonal antibody recognizes Occludin

反应物种 Hu, Ms
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Occludin
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminus of human occludin.
偶联标记 Un-conjugated
別名 PPP1R115; BLCPMG; Occludin


应用 推荐稀释比
WB1:25 - 1:500
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Lung, Human Kidney


形式 Liquid
纯化 Immunogen affinity purified
缓冲液 PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
抗菌剂 < 0.1% Sodium azide
稳定剂 1% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 100506658 Human OCLN

GeneID: 18260 Mouse OCLN

Swiss-port # Q16625 Human Occludin

Swiss-port # Q61146 Mouse Occludin

基因名称 OCLN
全名 occludin
背景介绍 This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
生物功能 May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. [UniProt]
细胞定位 Membrane
产品亮点 Related products:
Occludin antibodies; Occludin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Signaling Transduction antibody
预测分子量 59 kDa
翻译后修饰 Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

检测图片 (2) Click the Picture to Zoom In

  • ARG53386 anti-Occludin antibody WB image

    Western Blot: Kidney stained with Occludin antibody (ARG53386)

  • ARG53386 anti-Occludin antibody IHC-P image

    Immunohistochemistry: Human Lung stained with Occludin antibody (ARG53386)


nuts_pic      Average

anti-Occludin antibody



Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-Occludin antibody


Sample:Mouse kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC


Study of Magnesium Formulations on Intestinal Cells to Influence Myometrium Cell Relaxation.

WB / Human

Uberti Francesca et al.
Nutrients.,  (2020)




Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response.

WB, IHC-P / Human, Mouse

Yang Rui-Cheng et al.
J Neuroinflammation.,  (2019)




Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier.

IHC-P / Mouse

Yang R et al.
Oncotarget., 63839 - 63855 (2016)