ARG40144
anti-OVGP1 antibody
anti-OVGP1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes OVGP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | OVGP1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 107-137 of Human OVGP1. |
| 偶联标记 | Un-conjugated |
| 別名 | MUC9; Mucin-9; Oviductal glycoprotein; Estrogen-dependent oviduct protein; EGP; OGP; CHIT5; Oviductin; Oviduct-specific glycoprotein |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human placenta |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | OVGP1 |
| 全名 | oviductal glycoprotein 1, 120kDa |
| 背景介绍 | This gene encodes a large, carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites located within threonine, serine, and proline-rich tandem repeats. The gene is similar to members of the mucin and the glycosyl hydrolase 18 gene families. Regulation of expression may be estrogen-dependent. Gene expression and protein secretion occur during late follicular development through early cleavage-stage embryonic development. The protein is secreted from non-ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoan acrosomal regions. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development. [UniProt] |
| 细胞定位 | Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules. [UniProt] |
| 预测分子量 | 75 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG40144 anti-OVGP1 antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human fallopian tube was blocked with 3% BSA for 0.5 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG40144 anti-OVGP1 antibody at 1:25 for 1 hour at 37°C.
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ARG40144 anti-OVGP1 antibody WB image
Western blot: 20 µg of Human placenta lysate stained with ARG40144 anti-OVGP1 antibody at 1:2000 dilution.
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ARG40144 anti-OVGP1 antibody FACS image
Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG40144 anti-OVGP1 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
