ARG45698

anti-ORC5L antibody

anti-ORC5L antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes ORC5L
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 ORC5L
抗原物种 Human
抗原 Synthetic peptide corresponding to middle region of human ORC5L.
偶联标记 Un-conjugated
別名 ORC5; Origin Recognition Complex Subunit 5; PPP1R117; Orc5p; ORC5T; ORC5L; Protein Phosphatase 1, Regulatory Subunit 117; Origin Recognition Complex, Subunit 5 (Yeast Homolog)-Like; Origin Recognition Complex, Subunit 5 Homolog (Yeast); Origin Recognition Complex, Subunit 5-Like (Yeast); Origin Recognition Complex, Subunit 5

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.1-0.25 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 50 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 26429 Mouse ORC5

GeneID: 5001 Human ORC5

Swiss-port # O43913 Human Origin recognition complex subunit 5

Swiss-port # Q9WUV0 Mouse Origin recognition complex subunit 5

基因名称 ORC5
全名 Origin Recognition Complex Subunit 5
背景介绍 The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Oct 2010]
生物功能 Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. [UniProt]
细胞定位 Chromosome; Nucleus. [UniProt]
预测分子量 50 kDa
翻译后修饰 Ubl conjugation. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG45698 anti-ORC5L antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45698 anti-ORC5L antibody at 5 μg/ml dilution.

  • ARG45698 anti-ORC5L antibody WB image

    Western blot: MCF-7 stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.

  • ARG45698 anti-ORC5L antibody FACS image

    Flow Cytometry: U87 stained with ARG45698 anti-ORC5L antibody at 1 µg/10^6 cells dilution.

  • ARG45698 anti-ORC5L antibody WB image

    Western blot: Rat testis stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.

  • ARG45698 anti-ORC5L antibody WB image

    Western blot: Mouse testis stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.

  • ARG45698 anti-ORC5L antibody WB image

    Western blot: COS-7 stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.