ARG63132

anti-ORC3L antibody

anti-ORC3L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

概述

产品描述 Goat Polyclonal antibody recognizes ORC3L
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog
应用 IHC-P, WB
特异性 This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 ORC3L
抗原物种 Human
抗原 QKTDHVARLTWGGC
偶联标记 Un-conjugated
別名 LAT; Origin recognition complex subunit Latheo; ORC3L; LATHEO; Origin recognition complex subunit 3

应用说明

应用建议
应用 推荐稀释比
IHC-P3 - 5 µg/ml
WB1 - 3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 23595 Human ORC3

Swiss-port # Q9UBD5 Human Origin recognition complex subunit 3

背景介绍 The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
研究领域 Gene Regulation antibody
预测分子量 82 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG63132 anti-ORC3L antibody WB image

    Western Blot: HeLa lysate (RIPA buffer, 1.4E5 cells per lane) stained with ARG63132 anti-ORC3L antibody at 2 µg/ml dilution.

  • ARG63132 anti-ORC3L antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63132 anti-ORC3L antibody at 3.75 µg/ml dilution followed by AP-staining.