ARG40529
anti-OGDH antibody
anti-OGDH antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
参考文献2
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes OGDH |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | OGDH |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 148-427 of Human OGDH (NP_001003941.1). |
| 偶联标记 | Un-conjugated |
| 別名 | 2-oxoglutarate dehydrogenase complex component E1; EC 1.2.4.2; AKGDH; OGDC-E1; OGDC; 2-oxoglutarate dehydrogenase, mitochondrial; E1k; Alpha-ketoglutarate dehydrogenase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse brain | ||||||||
| 实际分子量 | 110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | OGDH |
| 全名 | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| 背景介绍 | This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] |
| 生物功能 | The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). [UniProt] |
| 细胞定位 | Mitochondrion matrix. Nucleus. Note=Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation. [UniProt] |
| 预测分子量 | 116 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG40529 anti-OGDH antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG40529 anti-OGDH antibody.
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ARG40529 anti-OGDH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG40529 anti-OGDH antibody at 1:100 dilution.
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ARG40529 anti-OGDH antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG40529 anti-OGDH antibody at 1:1000 dilution.
文献引用
The effect of ochratoxin A on cytotoxicity and glucose metabolism in human esophageal epithelium Het-1A cells
WB / Human
Ochratoxin A induces reprogramming of glucose metabolism by switching energy metabolism from oxidative phosphorylation to glycolysis in human gastric epithelium GES-1 cells in vitro.
WB / Human
