ARG65657
anti-OCT4 antibody
anti-OCT4 antibody for Western blot and Human,Mouse
Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes OCT4 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 靶点名称 | OCT4 |
| 抗原物种 | Mouse |
| 抗原 | Synthetic peptide around the internal region of Mouse OCT4 (C-DRPNAVKLEKVEP). |
| 偶联标记 | Un-conjugated |
| 別名 | OTF-3; OCT4; OCT3; Octamer-binding protein 4; OTF3; Oct-3; Oct-4; OTF4; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Octamer-binding protein 3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20263 Mouse POU domain, class 5, transcription factor 1 Swiss-port # Q01860 Human POU domain, class 5, transcription factor 1 |
|---|---|
| 基因名称 | Pou5f1 |
| 全名 | POU domain, class 5, transcription factor 1 |
| 背景介绍 | The protein encoded by this gene belongs to the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein plays a key role in embryonic development and stem cell pluripotency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. [UniProt] |
| 研究领域 | Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 38.2 kDa (NP_038661.2) |
| 翻译后修饰 | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. |
检测图片 (2) Click the Picture to Zoom In
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ARG65657 anti-OCT4 antibody WB image
Western blot: 35 μg of NIH3T3 lysate stained with ARG65657 anti-OCT4 antibody at 1 μg/ml dilution (1 hour incubation).
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ARG65657 anti-OCT4 antibody WB image
Western blot: 35 µg of HepG2 cell lysate (in RIPA buffer) stained with ARG65657 anti-OCT4 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
