ARG54001
anti-OCT4 antibody
anti-OCT4 antibody for Western blot and Mouse
Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes OCT3/OCT4 |
|---|---|
| 反应物种 | Ms |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | OCT4 |
| 抗原物种 | Mouse |
| 抗原 | Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | OTF-3; OCT4; OCT3; Octamer-binding protein 4; OTF3; Oct-3; Oct-4; OTF4; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Octamer-binding protein 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20263 Mouse POU domain, class 5, transcription factor 1 |
|---|---|
| 基因名称 | Pou5f1 |
| 全名 | POU domain, class 5, transcription factor 1 |
| 背景介绍 | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3').Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206.Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| 生物功能 | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. [UniProt] |
| 细胞定位 | Nucleus. |
| 研究领域 | Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. |
检测图片 (1) Click the Picture to Zoom In
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ARG54001 anti-OCT4 antibody WB image
Western blot: F9 cell lysate stained with ARG54001 anti-OCT4 antibody at 1:1000 dilution.
