ARG56993
anti-OAT antibody [23A2]
anti-OAT antibody [23A2] for Flow cytometry,ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [23A2] recognizes OAT |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 23A2 |
| 同位型 | IgG1, kappa |
| 靶点名称 | OAT |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 33-439 of Human OAT. |
| 偶联标记 | Un-conjugated |
| 別名 | OATASE; OKT; Ornithine aminotransferase, mitochondrial; Ornithine--oxo-acid aminotransferase; GACR; HOGA; Ornithine delta-aminotransferase; EC 2.6.1.13 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P04181 Human Ornithine aminotransferase, mitochondrial Swiss-port # P29758 Mouse Ornithine aminotransferase, mitochondrial |
|---|---|
| 基因名称 | OAT |
| 全名 | ornithine aminotransferase |
| 背景介绍 | This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010] |
| 预测分子量 | 49 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG56993 anti-OAT antibody [23A2] ICC/IF image
Immunoflorescense: HeLa cells stained with ARG56993 anti-OAT antibody [23A2] 1:500 (Red).
Hoechst 33342 (Blue) for nucleus staining.
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ARG56993 anti-OAT antibody [23A2] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56993 anti-OAT antibody [23A2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56993 anti-OAT antibody [23A2] WB image
Western blot: 35 µg of Mouse liver stained with ARG56993 anti-OAT antibody [23A2] at 1:1000.
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ARG56993 anti-OAT antibody [23A2] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG56993 anti-OAT antibody [23A2] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56993 anti-OAT antibody [23A2] WB image
Western blot: 1) 20 ng of OAT recombinant protein, 2) 40 ug of 293T cell lysate, and 3) 40 ug of Brain tissue lysate stained with ARG56993 anti-OAT antibody [23A2] at 1:1000.
