ARG63833
anti-OASIS / CREB3L1 antibody
anti-OASIS / CREB3L1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes OASIS / CREB3L1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | OASIS / CREB3L1 |
| 抗原物种 | Human |
| 抗原 | C-DFSHSKEWFHDRD |
| 偶联标记 | Un-conjugated |
| 別名 | Old astrocyte specifically-induced substance; OASIS; cAMP-responsive element-binding protein 3-like protein 1; Cyclic AMP-responsive element-binding protein 3-like protein 1 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96BA8 Human Cyclic AMP-responsive element-binding protein 3-like protein 1 |
|---|---|
| 背景介绍 | The protein encoded by this gene is normally found in the membrane of the endoplasmic reticulum (ER). However, upon stress to the ER, the encoded protein is cleaved and the released cytoplasmic transcription factor domain translocates to the nucleus. There it activates the transcription of target genes by binding to box-B elements. [provided by RefSeq, Jun 2013] |
| 研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2). RIP is induced by TGFB1 and ceramide (PubMed:25310401, PubMed:27499293). N-glycosylated. Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L1 is stabilized. |
检测图片 (3) Click the Picture to Zoom In
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ARG63833 anti-OASIS / CREB3L1 antibody WB image
Western Blot: A549 cell lysate stained with ARG63833 anti-OASIS / CREB3L1 antibody. Approx 60kDa band observed in lysates of cell line (calculated MW of 57.0kDa according to NP_443086.1). Recommended concentration: 1-3µg/ml.
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ARG63833 anti-OASIS / CREB3L1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63833 anti-OASIS / CREB3L1 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63833 anti-OASIS / CREB3L1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63833 anti-OASIS / CREB3L1 antibody at 3.75 µg/ml dilution followed by AP-staining.
