ARG22636

anti-O-GalNAc + O-GlcNAc antibody [9B9]

anti-O-GalNAc + O-GlcNAc antibody [9B9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other

概述

产品描述 Mouse Monoclonal antibody [9B9] recognizes O-GalNAc + O-GlcNAc
反应物种 Other
应用 ICC/IF, IHC-P, WB
特异性 Specific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
宿主 Mouse
克隆 Monoclonal
克隆号 9B9
同位型 IgM
靶点名称 O-GalNAc + O-GlcNAc
抗原物种 Others
抗原 Synthetic β-O-Linked N-Acetylglucosamine conjugated to KLH.
偶联标记 Un-conjugated

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50
IHC-P1:25
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 PEG Purified.
缓冲液 PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol.
抗菌剂 0.9% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (3) Click the Picture to Zoom In

  • ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] ICC/IF image

    Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] (green) at 1:50 dilution for 30 - 60 min at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.

  • ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse colon tissue stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] (green) at 1:25 dilution for 1 hour at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.

  • ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] WB image

    Western blot: 2.0 µg of GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, and Glucose-BSA stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] at 1:1000 for 2 hours at RT.