ARG66688
anti-Nur77 / TR3 phospho (Ser351) antibody
anti-Nur77 / TR3 phospho (Ser351) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Nur77 / TR3 phospho (Ser351) |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | The antibody detects Nur77 protein only when phosphorylated at Ser351. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Nur77 / TR3 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser351 of Human Nur77 / TR3. |
| 偶联标记 | Un-conjugated |
| 別名 | ST-59; N10; Nur77; GFRP1; Testicular receptor 3; NP10; NAK-1; HMR; Orphan nuclear receptor TR3; Nuclear hormone receptor NUR/77; Orphan nuclear receptor HMR; Early response protein NAK1; NGFIB; TR3; NUR77; Nuclear receptor subfamily 4 group A member 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 | ||||||
| 实际分子量 | ~ 64 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P22736 Human Nuclear receptor subfamily 4 group A member 1 |
|---|---|
| 基因名称 | NR4A1 |
| 全名 | nuclear receptor subfamily 4, group A, member 1 |
| 背景介绍 | This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| 生物功能 | Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. [UniProt] |
| 预测分子量 | 64 kDa |
| 翻译后修饰 | Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli. Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66688 anti-Nur77 / TR3 phospho (Ser351) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG66688 anti-Nur77 / TR3 phospho (Ser351) antibody. The picture on the right is blocked with the phospho peptide.
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ARG66688 anti-Nur77 / TR3 phospho (Ser351) antibody WB image
Western blot: HepG2 cell nucleus lysate stained with ARG66688 anti-Nur77 / TR3 phospho (Ser351) antibody.
