ARG56192
anti-Nucleolin antibody [364-5]
anti-Nucleolin antibody [364-5] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [364-5] recognizes Nucleolin |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat, Cow |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 364-5 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Nucleolin |
| 抗原物种 | Human |
| 抗原 | SUDHL1 cell nuclear lysate. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein C23; C23; Nucleolin |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NCL |
| 全名 | nucleolin |
| 背景介绍 | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] |
| 生物功能 | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt] |
| 细胞定位 | Nucleoli |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). |
检测图片 (4) Click the Picture to Zoom In
-
ARG56192 anti-Nucleolin antibody [364-5] FACS image
Flow Cytometry: Testing of 293T cells. Black: Cells alone; Grey: Isotype control; Green: AF488-conjugated ARG56192 anti-Nucleolin antibody [364-5].
-
ARG56192 anti-Nucleolin antibody [364-5] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skin stained with ARG56192 anti-Nucleolin antibody [364-5].
-
ARG56192 anti-Nucleolin antibody [364-5] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG56192 anti-Nucleolin antibody [364-5].
-
ARG56192 anti-Nucleolin antibody [364-5] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma stained with AF488-Conjugate ARG56192 anti-Nucleolin antibody [364-5].
