ARG40667

anti-Nrf 2 phospho (Ser40) antibody

anti-Nrf 2 phospho (Ser40) antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; NRF-2 Nuclear Localization antibody
publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes Nrf 2 phospho (Ser40)
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Nrf 2
抗原物种 Human
抗原 Phosphospecific peptide around Ser40 of Human Nrf 2.
偶联标记 Un-conjugated
別名 Nuclear factor, erythroid derived 2, like 2; NF-E2-related factor 2; Nuclear factor erythroid 2-related factor 2; HEBP1; NRF2; NFE2-related factor 2

应用说明

应用建议
应用 推荐稀释比
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:1000 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4780 Human NFE2L2

Swiss-port # Q16236 Human Nuclear factor erythroid 2-related factor 2

基因名称 NFE2L2
全名 nuclear factor, erythroid 2-like 2
背景介绍 This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]
生物功能 Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. [UniProt]
细胞定位 Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. [UniProt]
产品亮点 Related products:
Nrf 2 antibodies; Nrf 2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Keap1-Nrf2-ARE antibody panel is launched
研究领域 Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; NRF-2 Nuclear Localization antibody
预测分子量 68 kDa
翻译后修饰 Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.

Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG40667 anti-Nrf 2 phospho (Ser40) antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.

  • ARG40667 anti-Nrf 2 phospho (Ser40) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.

  • ARG40667 anti-Nrf 2 phospho (Ser40) antibody WB image

    Western blot: HepG2 cell lysate stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.

文献引用

Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis

WB, IHC-P / Human

Wei-Lun Hsu et al.
Cell Death Dis,  (2022)

publication_link

 

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